Peb Yuav Xaiv Qhov Twg? Iqf Qhov Dej Ua Nitrogen Lub Freezer lossis Khaub thuas Khaub Ncaws Khaub Ncaws

Khaub thuas txias txias thiab iqf qhov dej ua kua nitrogen lub freezer tau siv dav hauv kev ua kom khov sai ntawm txhua yam khoom lag luam hauv dej thiab khoom noj khov, kom khaws cov khoom noj muaj txiaj ntsig qub thiab xim thiab tsw ntawm cov zaub mov mus rau qhov siab tshaj plaws, thiab feem ntau siv nrog lub tub yees txias txias. Txawm hais tias muaj ntau yam ntawm lub cev thiab kev hloov pauv yuav tshwm sim hauv cov txheej txheem khov ntawm cov zaub mov, xws li ntim qis, nce kev noj kom qhuav, muaj protein ntau denaturation, hloov xim, hloov pauv lub neej thiab kev ua haujlwm micro-lub neej, tab sis cov txheej txheem khov sai ua rau cov kev hloov pauv saum toj no mus txog siab tshaj plaws reversibility Yog li, vim li cas txoj hauv kev no tuaj yeem khaws qhov zoo ntawm cov khoom noj khov sai sai yam tsis poob tom qab yaj.

Khaub thuas txias txias thiab iqf qhov dej ua kua nitrogen lub freezer tuaj yeem khov zaub mov hauv lub sijhawm luv luv kom lub hauv paus kub ntawm cov zaub mov nce mus txog -18 ° C. Ib lub sijhawm luv ntawm khov sai tuaj yeem zam kev tsim cov dej khov loj ntawm cov cell thiab txo kev sib cais ntawm cov dej hauv cov cell kom lub sijhawm sib cuag ntawm cov kuab tshuaj, cov ntaub so ntswg zaub mov, colloid, thiab ntau yam khoom sib xyaw hauv cov nqaij mos ntawm tes tau qis dua, thiab kev tsim txom ntawm kev mloog zoo kuj tseem txo qis. Nyob rau tib lub sijhawm, qhov chaw sov tsawg ntawm qhov txias txias cia thiab iqf qhov dej ua kua nitrogen lub tub yees lub tub yees ua haujlwm nyob rau hauv qhov kub thiab txias ntawm cov kab mob me me thiab cov enzymes, thiab nws txoj kev loj hlob thiab cov tshuaj tiv thaiv kab mob ua haujlwm tau zoo, uas tuaj yeem ua kom zoo dua qub khoom noj thiab txhim kho cov kab mob khaub thuas txias tom ntej.

Qhov ntau dua ntawm qhov txias txias cia txias thiab lub sijhawm khov sai yog luv dua, yog li lub zog ntawm cov khoom siv tub yees muaj zog dua, thiab tus nqi ntawm qhov ua kom khov sai dua yog siab dua.

Kev ua zaub mov khov-khov sai ntawm iqf qhov dej ua kua nitrogen lub freezer tau ua tiav los ntawm txoj siv hlua khi lossis phaj siv txoj siv thauj khoom kom cov khov khov yuav nrawm dua li khov khov txias cia.

Yog lawm, peb tuaj yeem xaiv raws li qhov xav tau txuas ntxiv ntawm cov khoom khov sai, uas tuaj yeem siv tsis tau tsuas yog khov txias txias tab sis tseem siv iqf qhov dej ua kua nitrogen lub freezer. Thaum peb siv lub iqf qhov dej ua kua nitrogen lub freezer kom khov zaub mov, peb tsuas yog xav kom cov khoom ntws mus rau hauv qhov av, muaj cua sov tas mus li, chav tub yees yuav ib txwm khiav, kom txog thaum cov khoom tso tseg, chav yuav cia li tso tseg. mus rau qhov kub nyob hauv qhov av. Qhov no tsis tsuas yog txhim kho kev siv tus nqi thiab kev ua haujlwm tau zoo ntawm cov cuab yeej, tab sis kuj tseem txuag lub zog siv hluav taws xob ntawm lub tsev vim kev pib nquag, thiab ua lub luag haujlwm txuag hluav taws xob.